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KMID : 0624620120450010032
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BMB Reports
2012 Volume.45 No. 1 p.32 ~ p.37
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The expression analysis of mouse interleukin-6 splice variants argued against their biological relevance
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Annibalini Giosue
Guescini Michele
Agostini Deborah
De Matteis Rita
Sestili Piero
Tibollo Pasquale
Mantuano Michela
Martinelli Chiara
Stocchi Vilberto
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Abstract
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Alternative splicing generates several interleukin-6 (IL-6) isoforms; for them an antagonistic activity to the wild-type IL-6 has been proposed. In this study we quantified the relative abundance of IL-6 mRNA isoforms in a panel of mouse tissues and in C2C12 cells during myoblast differentiation or after treatment with the Ca(2+) ionophore A23187, the AMP-mimetic AICAR and TNF-α. The two mouse IL-6 isoforms identified, IL-6δ5 (deletion of the first 58 bp of exon 5) and IL-6δ3 (lacking exon 3), were not conserved in rat and human, did not exhibit tissue specific regulation, were expressed at low levels and their abundance closely correlated to that of full-length IL-6. Species-specific features of the IL-6 sequence, such as the presence of competitive 3' acceptor site in exon 5 and insertion of retrotransposable elements in intron 3, could explain the production of IL-6δ5 and IL-6δ3. Our results argued against biological significance for mouse IL-6 isoforms.
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KEYWORD
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Alternative splicing, C2C12, Cytokines, Retrotransposons, Splicing error
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